SKU: 66936250750

Endoproteinase Glu-C

Sale price$140.40 Regular price$156.00
Save 10%

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 10 - Jul 15

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Endoproteinase Glu-CProduct Specification Species Staphylococcus aureus Synonyms Glutamyl endopeptidaseV8 proteaseEndoproteinase Glu CV8 proteinaseV8Glu C Expression System E. coli Molecular Weight 25kDa (Reducing) Purity 95% by SDS PAGE Tag His Tag Physical Appearance Lyophilized Powder Reconstitution To get the most use out the enzyme, resuspend the enzyme in 500 l H2O and aliquot 50 ul each in 10 tubes. Stability & Storage Store at 25 ~ 15 for 2 years Reference [1]

Product Specification


Species Staphylococcus aureus
Synonyms Glutamyl endopeptidase、V8 protease、Endoproteinase Glu-C、V8 proteinase、V8、Glu-C
Expression System E.coli
Molecular Weight

25kDa (Reducing)

Purity >95% by SDS-PAGE
Tag His Tag
Physical Appearance Lyophilized Powder
Reconstitution

To get the most use out the enzyme, resuspend the enzyme in 500 μl H2O and aliquot 50 ul each in 10 tubes.

Stability & Storage

Store at -25 ~ -15℃ for 2 years

Reference

[1] Yabuta M , Ochi N , Ohsuye K .Hyperproduction of a recombinant fusion protein of Staphylococcus aureus V8 protease in Escherichia coli and its processing by OmpT protease to release an active V8 protease derivative.[J].Appl Microbiol Biotechnol, 1995, 44(1-2):118-125.
[2] Prasad L , Leduc Y , Hayakawa K ,et al. The structure of a universally employed enzyme: V8 protease from Staphylococcus aureus[J].Acta Crystallographica Section D Biological Crystallography, 2004.

Background

Staphylococcus aureus V8 protease (Endoproteinase Glu-C) belongs to the serine protease family and can specifically hydrolyze carboxy-terminal peptide bonds of glutamic acid (Glu) or aspartate (Asp) residues. Its specificity is directly affected by the composition of the buffer, recognizing and cutting Glu carboxy-terminal peptide bonds in NH4HCO3 of pH7.8 and CH3COONH4 of pH4.0, recognizing and cutting Glu or Asp carboxy-terminal peptide bonds in phosphate buffers of pH7.8,but the hydrolysis rate of Glu was higher than that of Asp. Glu-C has been reported to be active in 0.2% SDS (sodium lauryl sulfate) and 4.0M urea.

Components

Supplied in dry format from Tris-HCl and sodium chloride buffer

Protocol

For the digestion of peptides or proteins, the ratio of enzyme to substrate between is 1:20 and 1:100 (w/w) for recommended. Dissolve the peptide or protein and was enzymolysis in 100 mM NH4HCO3, pH 7.8 or 100 mM Tris-HCl, pH 7.8, the recommended incubation time is 2-18 hours at 37°C, depending on the temperature and the ratio of enzyme to substrate.

Guidelines

Repeated freeze/thawing is not recommended.

Unit Definition

Using 2-Ph-leu-Glu-4-Na as substrate, the amount of enzyme required to produce 1μmol 4-Nitroaniline in 1 minute at pH7.8, 25℃ was 1unit.
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 66936250750

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.9 ★★★★★
Based on 646 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
J
Verified Purchase
John
Omaha, US
★★★★★ 4
Has fit perfectly into my morning espresso
Color: Black&Silver
This seems to me to be an excellent small kitchen scale. I am new to the complexities of espresso making and have begun using this scale every morning to measure my dose and output. It is working just fine. Easy to operate, keep clean and store. I did not give it 5 stars because I have limited comparisons but I don’t expect and will not bother looking for anything better.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
D
Verified Purchase
D. Lakin
Cuba, US
★★★★★ 5
Perfectly fits what I needed for my espresso habit!
Color: Black&Silver
This Maestri House mini coffee scale is a great little tool for espresso and pour-over. The size is compact enough to fit under a portafilter or cup, and the 0.1g accuracy makes dialing in coffee much easier. I like the built-in timer, rechargeable USB-C battery, and clean black-and-silver look. It feels precise, practical, and easy to keep near the espresso machine. Great value for anyone who wants more consistent coffee.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 2, 2026
B
Verified Purchase
B. Swenson
Bozeman, US
★★★★★ 5
Maestro House Mini Coffee Scale
I have begun to drink tea, pu’erh tea to be specific. I have several varieties and I have had a problem with getting consistent results. The varieties can be very different in size depending on when the leaves are harvested (leaves harvested in spring are much smaller). So the easiest way to get consistent results is by weight. This scale is very easy to use with a ceramic tea scoop, set the tea scoop on the scale, zero it out and add tea leaves. This has made my results much better, without the wild swings in strength I had been getting before trying to eyeball even measures. This scale gives me accurate measurements to one tenth of a gram.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 15, 2026
B
Verified Purchase
bicyclemayhem
Alexandria, US
★★★★★ 4
Mostly great, except one little annoyance
Color: Black&Silver, Color: Black&Silver
Pros- lightweight, small, very accurate, easy to clean, timer included. Cons- I use this to brew espresso on my Breville Touch and to get an accurate reading, you have to place something flat (structured) under the scale. Otherwise the reading just jumps all over the place. I know lot of espresso machines have the same base, so this might be an issue for some! This is my reason for 4/5 stars. It’s an easy fix, it’s just kind of annoying. Value for the price seems to be on point. It’s easy to clean. It has a timer as well as scale (nice for espresso). Buttons work the first time you push them. That’s it that’s all
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 31, 2026
E
Verified Purchase
Eglantine
Lowell, US
★★★★★ 5
Great taste
Flavor Name: Ceremony Matcha-1.06oz
Great quality matcha. It came with a cute little metal spoon. The color was bright green, just the way its supposed to be.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 17, 2026

recommand products